Phytologia Balcanica 32: 51-61, doi: 10.3897/phytolbalcan.32.e187443
Transferability and discriminating power of ISSR and SCoT markers for studying genetic diversity in Dacryodes edulis accessions from Nigeria
expand article infoTemitope Omoniyi Oyebola, Adeniyi Akanni Jayeola, Kafilat Abiodun Odesola, Temitope Esther Shonde§, Temitope Esther Owoeye|
‡ University of Ibadan, Ibadan, Nigeria§ International Institute of Tropical Agriculture, Ibadan, Nigeria| National Biotechnology Research and Development Agency, Abuja, Nigeria
Open Access
Abstract
Dacryodes edulis is a culturally and economically important fruit tree native to Central and West Africa. Despite its benefits, its genetic diversity remains underexplored. This study has used Inter-Simple Sequence Repeat (ISSR) and Start Codon Targeted (SCoT) markers to assess the genetic diversity and relationships among 32 accessions collected from Nigeria. Genomic DNA was extracted using a modified CTAB protocol and amplified by 15 ISSR and 10 SCoT primers. The results highlight the effectiveness of these markers in elucidating genetic variation and phylogenetic relationships, thus providing a foundation for genetic resource management and conservation.
Keywords
Genetic diversity, DNA markers, polymorphic information content
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